2 way flirt cam
Second, we isolated the pulsed-light effects from phenotypic traits of female flies by mounting one live female fly and one live male fly side by side (Fig.), immobilizing their wings and illuminating the wings of the male by the 190-Hz light pulses while keeping the wings of the paired female under constant illumination.Significantly more alighting responses by males on or near females that could move their wings than on females that could not (mean ± SE: 34.10 ± 3.76 vs.20.60 ± 3.79; n = 10, flies mounted on their abdominal ventrum to leave their legs without support and thus induce a wing fanning response.To test whether wing movement of females affects mate recognition by males, we mounted two live females side by side (Fig.
a–d Photographs in the upper row reveal changes in the intensity of light reflected off the wing as it rotates during wing fanning, thus causing a flashing light effect in b; e–h Photographs in the lower row fail to reveal any flashing light effect.wings mounted on hemostatic clamps and exposed to diffuse sunlight (a) and direct sunlight (b-f) on a day with periods of sunshine and clouds.In sub-panels b–f, note the bright sunlight reflected off the right wing in each pair : Figure S1a), one that produced light pulses at 190 Hz approximating the wing flash frequency of a flying female, and the other that produced constant light at the same intensity.Following each recording, which typically captured 1–6 flies in free flight, we proceeded to the next recording with a new set of flies in another cage.Analyzing the video-recorded data files (e.g.; Additional file 3: Video S1; Additional file 4: Video S2), we found differences in the frequency of light flashes reflected off the wings of free-flying young and old females and young and old males (one-way ANOVA; : Data S1).