Peptidylglycine alpha amidating enzyme Sex dating philippines
The nonenzymic conversion required no cofactor but was extremely accelerated by the addition of copper ion or at higher temperature.
Our data suggest that the direct product of the alpha-amidating reaction is not an alpha-amidated peptide but a hydroxyl derivative at the alpha-carbon of the carboxyl-terminal glycine.
In vertebrates, peptidylglycine alpha-amidating monooxygenase (PAM) is a multifunctional protein found in secretory granules.
The protein contains two enzymes, peptidylglycine alpha-hydroxylating monooxygenase (PHM) and peptidyl-alpha-hydroxyglycine alpha-amidating lyase (PAL), that act sequentially to catalyse the alpha-amidation of neuroendocrine peptides [PMID: 1988445, PMID: 1448112]: The product is unstable and dismutates to glyoxylate and the corresponding desglycine peptide amide.
With the purified enzyme, we detected an intermediate of the alpha-amidating reaction by high performance liquid chromatography analysis.
The production of the intermediate required copper, oxygen, and ascorbate and increased linearly with incubation time.
Peptidylglycines with a neutral amino acid residue in the penultimate position are the best substrates for the enzyme.
The product is unstable and dismutates to glyoxylate and the corresponding desglycine peptide amide, a reaction catalysed by EC 18.104.22.168 peptidylamidoglycolate lyase.
To restore access and understand how to better interact with our site to avoid this in the future, please have your system administrator contact [email protected] first step of the reaction is catalysed by peptidylglycine alpha-hydroxylating monooxygenase (PHM), and is dependent on copper, ascorbate and molecular oxygen; peptidyl-alpha-hydroxyglycine alpha-amidating lyase (PAL) catalyses the second step of the reaction [PMID: 1448112].PHM share protein sequence similarity with dopamine-beta-monooxygenases (DBH), a class of ascorbate-dependent enzymes that requires copper as a cofactor and uses ascorbate as an electron donor.Your access to the NCBI website at gov has been temporarily blocked due to a possible misuse/abuse situation involving your site.This is not an indication of a security issue such as a virus or attack.